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Genomics Inform > Volume 8(1); 2010 > Article
DOI: https://doi.org/10.5808/gi.2010.8.1.050   
Effects of Mercuric Chloride on Gene Expression in NRK-52E Cells.
Joon Ik Ahn, Si Yeon Baik, Moon Jeong Ko, Hee Jung Shin, Hye Joo Chung, Ho Sang Jeong
Pharmacological Research Division, Toxicological Evaluation Research Department, National Institute of Food and Drug Safety Evaluation, Seoul 122-704, Korea. hosa33@korea.kr
Abstract
Mercuric chloride, a model nephrotoxicant was used to elucidate time- and dose-dependent global gene expression changes associated with proximal tubular toxicity. Rat kidney cell lines NRK-52E cells were exposed for 2, 6 and 12 hours and with 3 different doses of mercuric chloride. Cell viability assay showed that mercuric chloride had toxic effects on NRK-52E cells causing 20% cell death (IC20) at 40micrometer concentration. We set this IC20 as high dose concentration and 1/5 and 1/25 concentration of LC20 were used as mid and low concentration, respectively. Analyses of microarray data revealed that 738 genes were differentially expressed (more than two-fold change and p<0.05) by low concentration of mercuric chloride at least one time point in NRK-52E cells. 317 and 2,499 genes were differentially expressed at mid and high concentration of mercuric chloride, respectively. These deregulated genes showed a primary involvement with protein trafficking (CAV2, CANX, CORO1B), detoxification (GSTs) and immunity and defense (HMOX1, NQO1). Several of these genes were previously reported to be up-regulated in proximal tubule cells treated with nephrotoxicants and might be aid in promoting the predictive biomarkers for nephrotoxicity.
Keywords: gene expression; mercuric chloride; nephrotoxicity; NRK-52E cells


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