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Genomics Inform > Volume 3(4); 2005 > Article
X-linked Gene Expression Profiles by RNAi-Mediated BRCA1 Knockdown in MCF7 Cells.
Min Ae Song, Jung Hoon Park, Hee Jeong Ahn, Jung Jae Ko, Suman Lee
1Functional Genomics Lab, Bundang Campus, Pochon CHA University College of Medicine, Sungnam-Si, Gyeonggi-Do 463-836, Korea.
2CHA Research Institute, Bundang Campus, Pochon CHA University College of Medicine, Sungnam-Si, Gyeonggi-Do 463-836, Korea.
3Department of Pathology, Bundang CHA Hospital, Pochon CHA University College of Medicine, Sungnam-Si, Gyeonggi-Do 463-836, Korea.
Abstract
Germ-line mutations of the BRCA1 gene confer an increased risk for breast and ovarian cancers. BRCA1 in female cells is directly related with the maintenance of the inactive X chromosome (Xi). The effect by the loss of the BRCA1 function on the X chromosome gene expression remains unclear in cancer cells. We attempted to investigate the expression pattern of the X-linked genes by performing BRCA1 knockdown via RNA interference in the MCF 7 breast cancer cell line. The transcriptional and translational levels of BRCA1 were decreased over 95% in the MCF 7 cells after BRCA1 knockdown. The expression patterns of one hundred ninety X-linked genes were profiled by the X chromosome-specific cDNA arrays. A total of seven percent of the X-linked genes (14/190) were aberrantly expressed by over 2-fold in the MCF7-BRCA1 knockdown cells, which contained two up-regulated genes (2/190, 1%) and 12 downregulated genes (12/190, 6.3%). It is interesting that 72% of the aberrantly expressed X-linked genes were located on the Xq (10/14,) region. Our data suggests that BRCA1 may not be important to maintain X chromosome inactivation in cancer because the BRCA1 knockdown did increase the expression of the only one percent of X-linked genes in the human breast cancer cells.
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