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Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing |
Minho Lee, Shin-Jung Choi, Sangjo Han, Miyoung Nam, Dongsup Kim, Dong-Uk Kim, Kwang-Lae Hoe |
Genomics Inform. 2018;16(2):22-29. Published online June 30, 2018 DOI: https://doi.org/10.5808/GI.2018.16.2.22 |
Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing Deletion of the MAG1 DNA glycosylase gene suppresses alkylation-induced killing and mutagenesis in yeast cells lacking AP endonucleases Molecular biology of DNA repair in the fission yeast Schizosaccharomyces pombe Gene Deletion by Synthesis in Yeast Massively Parallel Sequencing Detected a Mutation in theMFN2Gene Missed by Sanger Sequencing Due to a Primer Mismatch on an SNP Site Unexpected insertion of carrier DNA sequences into the fission yeast genome during CRISPR–Cas9 mediated gene deletion Yeast sequencing reports.Gcs1, a gene encoding γ-glutamylcysteine synthetase in the fission yeastSchizosaccharomyces pombe Quick screening of plasmid deletion clones carrying inserts of desired sizes for DNA sequencing Towards Replacement of Sanger Sequencing with Next-Generation Sequencing in the Clinical Laboratory Construction of Exonuclease III Generated Nested Deletion Sets for Rapid DNA Sequencing |